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Porous polysaccharide-based scaffolds for human endothelial progenitor cells

Porous polysaccharide-based scaffolds for human endothelial progenitor cells

Human endothelial colony-forming cells (ECFCs) contribute to vascular repair. For this reason, they are considered as valuable cell therapy products in ischemic diseases. Cells used in this article are commercialized by ABCell-Bio society (reference: PEC) and isolated from cord blood. We have developed porous scaffolds composed of natural polysaccharides, pullulan and dextran, by chemical cross-linking without organic solvent. These porous scaffolds, which have pores with an average size of 42 m and a porosity of 21%, allow the viability and the proliferation of cord blood ECFCs. After 7 days of culture in porous scaffolds, ECFCs express endothelial markers (CD31 and vWf) and maintain endothelial functions. The cultured cells can be easily retrieved by enzymatic degradation of the porous scaffolds. In vitro results suggest that the porous scaffold could allow cell delivery of ECFCs for treatment of vascular diseases.

Authors: Mlanie Lavergne*, Mohammed Derkaoui, Catherine Delmau*, Didier Letourneur, Georges Uzan, Catherine Le Visage

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Date 14 February 2017
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